pituitary tumor cell gh3  (ATCC)

Journal: Endocrinology

Article Title: miR-375 Regulation of SSTR2 Expression in Corticotroph Pituitary Cells: Somatostatin Receptor Ligands Effects

doi: 10.1210/endocr/bqaf107

Figure Lengend Snippet: (A) Expression of circulating miR-375 in sera of 17 normal subjects and 26 patients with CD. (B) Expression of miR-375 in 2 normal pituitaries and 6 corticotroph pituitary adenomas tissues. (C) Expression of miR-375 in GH3 and AtT20/D16 cell lines. (D) Expression of miR-375 in AtT20/D16 cell line under DEX 10 −8 M treatment after 24 hours, 48 hours and 6 days. (E) Sstr2 gene expression in GH3 and AtT20/D16 cell lines. (F) Sstr2 gene expression modulation by DEX 10 −8 M treatment after 24 hours, 48 hours, and 6 days in AtT20/D16 cell line. Data in the graphs performed in cell lines represent mean ± SEM of 3 independent experiments. * P < .05, ** P < .01, *** P < .001, **** P < .0001 vs control or among the groups.

Article Snippet: AtT20/D16 cell line (CRL-1795), a strain of the mouse corticotroph pituitary tumor model AtT20, and GH3 cell line (CCL-82.1), a rat somatotroph pituitary tumor model, were provided by American Type Culture Collection (ATCC) and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, code number 16140071), 200 mM L-glutamine (Gibco, code number 25030081) and 1 × 10 5 U/L penicillin and streptomycin (Gibco, code number 15140122).

Techniques: Expressing, Gene Expression, Control